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Merck
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Key Documents

AB747

Sigma-Aldrich

Anti-Collagen Type III Antibody

Chemicon®, from rabbit

Sinónimos:

Anti-EDS4A, Anti-EDSVASC, Anti-PMGEDSV

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable

suitability

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... COL3A1(1281)

Specificity

The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.



Cross Reactivity Percent

Human collagen type I 10%

Human collagen type II 2%

Human collagen type III 100%

Human collagen type IV and V 4%



Human plasma proteins do not interfere with binding to collagen.

Immunogen

Human placental collagen type III

Application

ELISA 1:3,000

Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40

Optimal working dilutions must be determined by the end user.
This Anti-Collagen Type III Antibody is validated for use in ELISA, IF for the detection of Collagen Type III.

Physical form

500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.

Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Format: Purified

Storage and Stability

Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Optional

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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T K van den Berg et al.
The American journal of pathology, 143(4), 1098-1110 (1993-10-01)
In this study, we describe the immunocytochemical distribution of the beta 1 integrins (alpha 1 to alpha 6) and their extracellular matrix ligands in human peripheral lymphoid tissues. The results show that within these tissues individual beta 1 integrins are
Pallavi Bhatta et al.
mAbs, 13(1), 1859049-1859049 (2021-01-26)
Bispecific antibodies can uniquely influence cellular responses, but selecting target combinations for optimal functional activity remains challenging. Here we describe a high-throughput, combinatorial, phenotypic screening approach using a new bispecific antibody target discovery format, allowing screening of hundreds of target
Omar S Qureshi et al.
Biology open, 6(10), 1423-1433 (2017-10-17)
Activated fibroblasts are considered major drivers of fibrotic disease progression through the production of excessive extracellular matrix (ECM) in response to signals from damaged epithelial and inflammatory cells. Nevertheless, epithelial cells are capable of expressing components of the ECM, cross-linking
John A Marwick et al.
SLAS discovery : advancing life sciences R & D, 26(9), 1091-1106 (2021-06-04)
Lung imaging and autopsy reports among COVID-19 patients show elevated lung scarring (fibrosis). Early data from COVID-19 patients as well as previous studies from severe acute respiratory syndrome, Middle East respiratory syndrome, and other respiratory disorders show that the extent
Linghong Huang et al.
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Fibrotic remodeling is the primary driver of functional loss in chronic kidney disease, with no specific anti-fibrotic agent available for clinical use. Transglutaminase 2 (TG2), a wound response enzyme that irreversibly crosslinks extracellular matrix proteins causing dysregulation of extracellular matrix

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