AB1987
Anti-Neurofilament M (145 kDa) Antibody, CT
serum, Chemicon®
Sinónimos:
Anti-NEF3
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
reptile, chicken, rat, quail, pig, human, mouse, bovine, rabbit
fabricante / nombre comercial
Chemicon®
técnicas
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
bovine ... Nefm(281347)
chicken ... Nefm(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rabbit ... Nefm(100009343)
rat ... Nefm(24588)
Descripción general
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Especificidad
Strong and specific staining for NF-M (145 kDa)
Inmunógeno
Epitope: C-terminus
Recombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M
Aplicación
Anti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB.
Immunohistochemistry:
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Immunohistochemistry(paraffin):
Optimal working dilutions must be determined by end user.
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Immunohistochemistry(paraffin):
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Calidad
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..
Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..
Descripción de destino
145 kDa
Forma física
Neat rabbit antiserum containing no preservative.
Unpurified
Almacenamiento y estabilidad
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve
Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Axons are injured by antigen-specific CD8(+) T cells through a MHC class I- and granzyme B-dependent mechanism.
Sauer, BM; Schmalstieg, WF; Howe, CL
Neurobiology of Disease null
Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses.
Alami, NH; Jung, P; Brown, A
The Journal of Neuroscience null
L J Ellett et al.
Scientific reports, 6, 30269-30269 (2016-07-30)
Patients with Parkinson's disease often experience non-motor symptoms including constipation, which manifest prior to the onset of debilitating motor signs. Understanding the causes of these non-motor deficits and developing disease modifying therapeutic strategies has the potential to prevent disease progression.
Zhenzhong Ma et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(26), 9630-9640 (2011-07-01)
During peripheral nervous system development, successful communication between axons and Schwann cells is required for proper function of both myelinated and nonmyelinated nerve fibers. Alternatively spliced proteins belonging to the neuregulin1 (NRG1) gene family of growth and differentiation factors are
GSK3B-mediated phosphorylation of MCL1 regulates axonal autophagy to promote Wallerian degeneration.
Shuji Wakatsuki et al.
The Journal of cell biology, 216(2), 477-493 (2017-01-06)
Macroautophagy is a catabolic process, in which portions of cytoplasm or organelles are delivered to lysosomes for degradation. Emerging evidence has indicated a pathological connection between axonal degeneration and autophagy. However, the physiological function and induction mechanism of autophagy in
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