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Reactivo de transfección GeneJuice®

GeneJuice® Transfection Reagent
1 of 1 reviewers received a sample product or took part in a promotion

Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.

Sinónimos:

Reactivo de transfección, Transfección GeneJuice

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About This Item

Código UNSPSC:
41106502
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

Formulario

liquid

fabricante / nombre comercial

Novagen®

condiciones de almacenamiento

OK to freeze

técnicas

transfection: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

«Utilizo GeneJuice porque es fácil de utilizar, tiene baja toxicidad y una amplia variedad de células diana»
Stuart Rulten. Research Fellow. Universidad de Sussex

«Desde que trabajamos con GeneJuice, hemos ahorrado mucho tiempo y dinero»
Dr. Andrea Kress, Instituto de biología clínica y molecular, Erlangen, Alemania

«Me parece que Genejuice funciona para muchos tipos de células con una optimización mínima»
Caitriona Marie Lyons. Estudiante de doctorado University College Cork, Irlanda

La transfección es el proceso mediante el cual se introducen ácidos nucleicos en células de mamífero. El reactivo de transfección GeneJuice es una formulación patentada optimizada para una máxima eficiencia de la transfección, facilidad de uso y mínima citotoxicidad para las células de mamífero. Mientras que muchos de los reactivos de transfección disponibles se basan en la formulación lipídica catiónica, el reactivo de transfección GeneJuice está compuesto por una proteína celular no tóxica y una pequeña cantidad de una nueva poliamina. El reactivo de transfección GeneJuice permite una transferencia muy eficiente de ADN en transfecciones estables y transitorias de células eucariotas y es ideal para transfecciones de gran rendimiento en un formato de placa multipocillo. Su composición exclusiva es compatible con medios que contienen suero y medios que no lo contienen, lo que hace que sea innecesario el cambio de medios durante los experimentos de transfección. Genejuice es una alternativa superior a una amplia variedad de otras técnicas, como la coprecipitación de fosfato de calcio, la electroporación, la microinyección, la administración de partículas biolísticas, la lipofección y la formación de complejos con DEAE-dextrano. El tamaño de 1 ml proporciona suficiente reactivo para realizar hasta 500 transfecciones en placas convencionales de 35 mm.


Líneas celulares transfectadas con GeneJuice® Reactivo de transfección
Líneas celulares:células primarias:
10T1/2

293T

3T3 NIH

3T3 Swiss

3T3-L1

A204

A431

A549

alfa TC1-6

AR 42J

As4.1

AtT-20

B50

BC-1

BC-2

BC3

BCBL

BHK-21

C3H/10T1/2

C6

C2C12
Caco-2

Caki-1

Calpan-1

Calu-1

Calu-6

CCL-131

CFPAC-1

Chang Liver

CHO

CHO-7

CHO-IR

CHO-K1

COS-1

COS-7

CS-1

CV-1

Daudi

DDTI MF-2

DT40

ECV304

EL4
ES-E14TG2a

EVSCC17M

H9c2

HCT-116

HEK293

HeLa

HeLa B

HeLa T4

Hep 3B2.1-7

HepG2

Hepa 1-6

Ht-29

HTB-37

HTB-45

Huh-7

HUVEC

IC21

IEC-6

JEG-3

Jurkat

KB
L57-3-11

L-6

L-929

MA-10

McA-RH7777

MCF-7

MCF-10-2A

MDCK

Melanocito

MG-63

Neuro 2A

Neuroblastoma

NRK

NT2/D1

OV-1063

OVCAR3

P4

P19

PC12

PA317

PAM212
PS-1

R2C

RAW 264.7

RBL-2H3

RMP-41

SAOS-2

SC-1

Schneider line2

SK-N-MC

SK-N-SH

SKOV3

STO

SW-480

SW-837

T3M4

TM4

U937

UCD

Vero

Células de músculo liso aórtico

Astrocitos

Angioblastos

Condrocitos

Células cromafines

Células epiteliales:

mamarias

prostáticas

traqueales

Fibroblastos

Queratinocitos

Reactivo de transfección química no lipídico optimizado para una máxima eficiencia de la transfección y facilidad de uso, y una mínima citotoxicidad en una amplia variedad de células de mamífero.

Características y beneficios

  • Transferencia de ADN muy eficiente para transfecciones estables y transitorias
  • Compatibilidad con medios que contienen suero y medios que no contienen suero
  • Protocolo sencillo, sin necesidad de cambiar medios
  • Ideal para transfección de gran rendimiento en formato de placa multipocillo
  • Ideal para la producción de retrovirus para la transducción de células T
  • Funciona para muchos tipos de células con una optimización mínima
  • Proporciona una eficiencia de transfección mayor y una citotoxicidad menor que los reactivos de otros proveedores
  • El tamaño de 1 ml proporciona suficiente reactivo como para realizar hasta 500 transfecciones en placas convencionales de 35 mm

Advertencia

Toxicidad: Inflamable (J)

Nota de preparación

Si desea ver el protocolo, pulse aquí

Otras notas

Debido a la naturaleza de los materiales peligrosos de este envío, podrían aplicarse gastos de envío a su pedido. Ciertos tamaños pueden estar exentos de los gastos de envío añadidos a los materiales peligrosos. Consulte a su oficina local de ventas si desea más información relativa a dichos gastos.

Información legal

GeneJuice® es una marca registrada de EMD Chemicals Inc.
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

FlameExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

67.1 °F - Information taken from reference works and the literature.

Punto de inflamabilidad (°C)

19.5 °C - Information taken from reference works and the literature.


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Questions

1–8 of 8 Questions  
  1. Can I use the product in the presence of serum?

    1 answer
    1. Yes. Our nucleic acid transfection reagents are effective for transfecting cells in media with or without serum. While cells can be incubated in media containing serum, it is absolutely critical that serum is NOT present during formation of the transfection reagent/DNA complex. For most applications, we recommend adding the transfection reagent/DNA complex (formed in serum-free media) to cells grown in complete growth media. For certain cell lines and experimental conditions, serum starvation of cells might be required. Since serum provides growth factors and nutrients, transfection efficiencies achieved with growth in serum containing media are typically better than those in serum-free media.

      Helpful?

  2. Why do I need to perform optimization? How should I go about doing it?

    1 answer
    1. Each cell type behaves differently, by carrying out an optimization, the best transfection condition for your particular cell type can be determined. In other words, you can avoid putting too much transfection reagent on your cells, which may cause unnecessary toxicity issue and waste of precious transfection reagent. Optimization is suggested for every new combination of cell type and plasmid. The most important parameters are cell density and ratio of transfection reagent to DNA. Start with the volume of the selected transfection reagent (1x) and plasmid amount (1x) as recommended in the User Protocol. If those conditions do not yield the desired results, an optimization experiment can be performed. In a 24-well plate, plate the same amount of cells in each well. Set up a gradient across the plate and add the appropriate volume of transfection reagent (0.5x, 1x, 1.5x, 2x, 2.5x and 3x). Set up a gradient down the plate and add the appropriate amount of plasmid (0.5x, 1x, 1.5x and 2x). With a reporter gene in the plasmid, the optimal condition can be easily determined.

      Helpful?

  3. How long should I leave the transfection reagent on the cells? Do I need to change medium at any time after transfection?

    1 answer
    1. Since our nucleic acid transfection reagents are compatible with serum-containing media, medium change after transfection is not necessary. The majority of cell types can be incubated with the transfection mix for 24-72h without any media change, and then harvested for the desired downstream application. If media change is necessary due to the toxicity of the protein being expressed, the transfection mixture can be removed after 2-8 h of incubation and replaced with complete growth medium.

      Helpful?

  4. Is the quality of DNA important for good transfection?

    1 answer
    1. Yes, it is essential that the DNA to be transfected is of high quality and free of endotoxins. Plasmid DNA preparations should include an endotoxin removal step.

      Helpful?

  5. How do I scale my transfection protocol when working with different culture volumes?

    1 answer
    1. For most standard culture formats, guidelines are provided in the User Protocol. If you are using different culture volumes, vary the amounts of DNA, transfection reagent, cells, and culture media in proportion to the relative surface area while keeping the transfection reagent: DNA ratio constant.

      Helpful?

  6. What is the size limit for plasmid DNA?

    1 answer
    1. Large plasmids in the range of 12-15 kb can be transfected. We have cloned and expressed inserts encoding large proteins (including β-gal) without difficulty in mammalian cell lines.

      Helpful?

  7. Will antibiotics interfere with transfection?

    1 answer
    1. We do not recommend including antibiotics during the formation of the transfection reagent/DNA complex. Increased cell permeability during transfection causes high antibiotic influx, resulting in cell death. Some antibiotics (such as kanamycin) are cationic and can therefore interfere with transfection. Antibiotics such as penicillin and streptomycin can be present in the complete growth media (with serum) which is used to grow the cells. If you are generating stable transfectants, add selection antibiotics (e.g., G 418 or hygromycin) 48-72h after transfection.

      Helpful?

  8. Can the DNA transfection reagents be used for co-transfecting plasmids?

    1 answer
    1. Yes. Multiple plasmids can be transfected into the cell at the same time. The key is to maintain the optimal ratio of total DNA (all plasmids). See the User Protocols for more information on the ratio of reagent to DNA.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. Oxford, UK
    • Review 1
    • Votes 0
    5 out of 5 stars.

    The best transfection reagent bar none

    GeneJuice is my go-to transfection reagent. I find it gives better transfection efficiencies with less DNA than lipid-based transfection reagents, and is well tolerated by every cell line I have tested it with.

    Helpful?

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