612200-M

Tissue Plasminogen Activator, Human

• Sinónimos: tPA
• Código UNSPSC: 12352202

Propiedades

• Ensayo: ≥95% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: lyophilized
• actividad específica: ≥300 KU/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca²⁺ antagonist may increase levels of tPA.

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween^®-80 Detergent.

Nota de preparación

Prepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Reconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).

Otras notas

Kim, Y.H., et al. 1999. Science284, 647.
Tsirka, S.E., et al. 1995. Nature 377, 340.
Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable