251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

• Sinónimos: DAF-2, 4,5-Diaminofluorescein
• Fórmula empírica (notación de Hill): C₂₀H₁₄N₂O₅
• Número de CAS: 205391-01-1
• Peso molecular: 362.34
• Número MDL: MFCD05865100
• Código UNSPSC: 12171500
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥98% (HPLC)
• Formulario: solution
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: pale yellow
• fluorescencia: λ_ex ~495 nm; λ_em ~515 nm
• temp. de almacenamiento: −20°C
• cadena SMILES: Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O
• InChI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• Clave InChI: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

Descripción

Descripción general

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Acciones bioquímicas o fisiológicas

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Advertencia

Toxicity: Irritant (B)

Forma física

A 5 mM (1 mg in 550 µl) solution in DMSO.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Información legal

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Punto de inflamabilidad (°C): 87 °C - closed cup - (Dimethylsulfoxide)