234109-M

Colcemid

Cell synchronization agent.

• Sinónimos: Colcemid, Demecolcine
• Fórmula empírica (notación de Hill): C₂₁H₂₅NO₅
• Número de CAS: 477-30-5
• Peso molecular: 371.43
• MDL number: MFCD00075459
• UNSPSC Code: 12352200

Propiedades

• assay: ≥98% (HPLC)
• form: crystalline powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: chloroform: 10 mg/mL; ethanol: soluble
• storage temp.: 10-30°C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
• InChI key: NNJPGOLRFBJNIW-UHFFFAOYSA-N

Descripción

General description

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Biochem/physiol Actions

Primary Target
Depolymerizes microtubules and limits microtubule formation.

Warning

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany