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Merck
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Key Documents

07-507

Sigma-Aldrich

Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody

Upstate®, from rabbit

Sinónimos:

Anti-Anti-BMK1, Anti-Anti-ERK4, Anti-Anti-ERK5, Anti-Anti-PRKM7

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, bovine, human

manufacturer/tradename

Upstate®

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

isotype

IgG

NCBI accession no.

NM_139033

UniProt accession no.

Q13164

shipped in

wet ice

target post-translational modification

phosphorylation (pThr218/pTyr220)

Gene Information

bovine ... Mapk7(537703)
human ... MAPK7(5598)
mouse ... Mapk7(23939)
rat ... Mapk7(114509)

Specificity

Recognizes phospho-BMK1/Erk5 (Thr218, Tyr220).

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5

Application

Peptide Inhibition Analysis:
HeLa cell lysate, HeLa cell lysate+ non-phosphorylated protein, and HeLa cell lysate + phosphorylated peptide were probed with Cat. No. 07-507, Anti-phospho-BMK 1/ERK5 (Thr218/Tyr220) (1µg/mL(1:1000) dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminesence detection system.

Arrow indicates BMK1/ERK5 (~115 kDa) on HeLa cell lysate & HeLa cell lysate + non-phosphorylated peptide. No band is present on the HeLa cell lysate + phosphorylated peptide indicating successful peptide inhibition of the antibody.
Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-phospho-BMK1/Erk5 (Thr218/Tyr220) Antibody is validated for use in WB for the detection of phospho-BMK1/Erk5 (Thr218/Tyr220).

Quality

Evaluated by Western Blotting/Peptide Inhibition Analysis in HeLa cell lysate.

Western Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected phospho-BMK1/Erk5 (Thr218/Tyr220) in HeLa cell lysate.

Target description

110-115kDa

Physical form

Affinity purified immunoglobulin in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.
ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at 2-8°C from date of shipment.

Analysis Note

Control
Sorbitol-treated HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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G Zhou et al.
The Journal of biological chemistry, 270(21), 12665-12669 (1995-05-26)
We have identified two components of a new protein kinase signaling cascade, MAPK/ERK kinase 5 (MEK5) and extracellular signal-regulated kinase 5 (ERK5). The MEK5 cDNA was isolated by degenerate PCR and encodes a 444-amino acid protein, which has approximately 40%
Kazuhiro Nakamura et al.
Methods in molecular biology (Clifton, N.J.), 661, 91-106 (2010-09-03)
Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1) or MAPK7. ERK5 is 115 kDa in mass and therefore larger than the other MAPKs such as ERK1/2, JNK, and p38. Like other MAPKs, ERK5 is ubiquitously expressed
Shan Jiang et al.
BioMed research international, 2017, 6895730-6895730 (2017-05-26)
Background. The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms. Methods. HUVECs were cultured and stimulated with different
Zeynep Madak-Erdogan et al.
Molecular cancer research : MCR, 12(5), 714-727 (2014-02-08)
Cancer cell motility and invasiveness are fundamental characteristics of the malignant phenotype and are regulated through diverse signaling networks involving kinases and transcription factors. This study establishes an estrogen receptor (ERα)/MAPK (ERK5)/cofilin (CFL1) network that specifies the degree of breast
Cathleen R Carlin et al.
Molecular biology of the cell, 34(12), ar116-ar116 (2023-08-30)
The polarized distribution of membrane proteins into apical and basolateral domains provides the basis for specialized functions of epithelial tissues. The EGF receptor (EGFR) plays important roles in embryonic development, adult-epithelial tissue homeostasis, and growth and survival of many carcinomas.
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