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Merck
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Documentos clave

07-1389

Sigma-Aldrich

Anti-phospho-Smad3 (Ser423/425) Antibody

from rabbit

Sinónimos:

MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mothers against DPP homolog 3 , SMA- and MAD-related protein 3, SMAD 3 , SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD, mothers

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

unpurified

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

mouse, mink

reactividad de especies (predicha por homología)

human, rat

técnicas

western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pSer423/pSer425)

Información sobre el gen

human ... SMAD3(4088)

Descripción general

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

Especificidad

Detects phosphorylated Smad3 (Ser423 and Ser425).

Inmunógeno

Epitope: Phosphorylated Ser423/425
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3.

Aplicación

Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB.
Research Category
Signaling

Calidad

Evaluated by western blot on untreated and UV-treated AKR-2B cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect Smad3 in UV-treated AKR-2B cell lysate.

Descripción de destino

50 kDa

Forma física

Unpurified rabbit serum in buffer containing 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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