Dye content is determined by back calculation from the carbon or nitrogen content, while purity is established by HPLC. The dye content is 60%. The impurities are not identified by either dye content or by methods such as HPLC, and no testing for impurities is conducted in this case. Both dye content and dye purity are determined for this product, with the Specification sheet listing minimum specifications for all lots, and individual Certificates of Analysis reporting lot-specific results. Water content is not measured, and the product is not considered hygroscopic.
302643
Trypan Blue
Dye content 60 %, ≥80% (HPLC)
Sinónimos:
Direct blue 14
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About This Item
Fórmula lineal:
C34H24N6O14S4Na4
Número de CAS:
Peso molecular:
960.81
Número de índice de color:
23850
Beilstein:
4360496
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nivel de calidad
Ensayo
≥80% (HPLC)
Formulario
powder
composición
Dye content, 60%
técnicas
microbe id | staining: suitable
color
dark green to brown, or Brown-Black
mp
>300 °C (lit.)
solubilidad
H2O: 1 mg/mL
λmáx.
607 nm
ε (coeficiente de extinción)
≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
room temp
cadena SMILES
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
Clave InChI
GLNADSQYFUSGOU-UHFFFAOYSA-J
Información sobre el gen
human ... PTPN1(5770) , PTPRF(5792)
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Descripción general
Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.
Aplicación
Acciones bioquímicas o fisiológicas
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
- to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
- to stain plant tissue in order to visualize cell death.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Carc. 1B
Código de clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 3
Equipo de protección personal
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Are the descriptions of Product 302643 as "Trypan Blue - 60% Dye content" and the purity limit of ">= 80.0%" on the Specification sheet and CofA referring to the same thing? If they are, why are they different? If not, should both be considered when aiming to achieve a specific dye concentration? Additionally, if it is 60% dye, what makes up the remaining 40%? Should the significant water content in this product be taken into consideration?
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