DEPC can be solubilized in 95% ethanol, e.g. 1.6 mL in 4 mL ethanol gives a clear, colorless solution. DEPC is soluble in alcohols, esters, ketones and other hydrocarbons, but has limited solubility in water (~0.1%). 6 DEPC hydrolyzes in aqueous solution, to form ethanol and carbon dioxide (CO2). Typically a 0.1% DEPC solution (1 mL of DEPC diluted in water to 1 L) is used to inactivate RNase. The DEPC will not immediately dissolve, as evidenced by the appearance of globules. The mixture should be stirred until the globules disappear. DEPC is sensitive to pH. In phosphate buffer at 25 °C, its half-life is 4 minutes at pH 6, and 9 minutes at pH 7. Hydrolysis is accelerated by Tris buffer,7 where at 25 °C, the half-life of DEPC was reported to be 1.25 minutes at pH 7.5, and 0.37 minutes at pH 8.2. DEPC will decompose in solution when autoclaved. For 0.1% DEPC solutions, autoclaving for 15 minutes per liter should be sufficient.
DEPC is very sensitive to moisture. As such, DEPC is packaged under argon to help reduce exposure to moisture. If DEPC is exposed to even traces of moisture, some hydrolysis occurs. The resulting CO2 is more soluble in DEPC solutions at 2–8 °C than at room temperature. As the product is brought to room temperature before opening, the DEPC can become supersaturated with respect to any dissolved gas. After opening the bottle for the first time and each time thereafter, layer nitrogen or argon gas over the DEPC and store the closed bottle at 2–8 °C for optimal stability. It may be helpful to store the bottle inside a sealed plastic bag with desiccant, but with the bottle cap slightly loose. If the bag inflates at all, this indicates some degree of decomposition with possible pressure build-up. Once opened, the bottle should not be kept for more than 9 months. DEPC decomposes at 155 °C. DEPC is also sensitive to ammonia, which causes decomposition to urethane.
159220
Dietilpirocarbonato
96% (NT)
Sinónimos:
DEP, DEPC, Dietildicarbonato, Dietiloxidiformato, Ácido etoxifórmico anhidro
About This Item
Productos recomendados
Nivel de calidad
Ensayo
96% (NT)
Formulario
liquid
índice de refracción
n20/D 1.398 (lit.)
bp
93-94 °C/18 mmHg (lit.)
densidad
1.101 g/mL at 25 °C (lit.)
grupo funcional
carbonate
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
cadena SMILES
CCOC(=O)OC(=O)OCC
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
Clave InChI
FFYPMLJYZAEMQB-UHFFFAOYSA-N
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Descripción general
Aplicación
Acciones bioquímicas o fisiológicas
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
156.2 °F - closed cup
Punto de inflamabilidad (°C)
69 °C - closed cup
Equipo de protección personal
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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