Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

RESUMEN

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

MATERIALES

Referencia del producto

Marca

Descripción del producto

695092

Sigma-Aldrich

Ácido acético, glacial, ACS reagent, ≥99.7%

A6283

Sigma-Aldrich

Ácido acético, glacial, ReagentPlus^®, ≥99%

M7506

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Sulfato de magnesio, anhydrous, ReagentPlus^®, ≥99.5%

360473

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2-Butanona, ACS reagent, ≥99.0%

208094

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Sulfato de magnesio, anhydrous, reagent grade, ≥97%

338826

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Ácido acético, glacial, ≥99.99% trace metals basis

213225

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Bromuro de litio, ReagentPlus^®, ≥99%

45754

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Ácido acético solution, suitable for HPLC

27225

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Ácido acético, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%

34861

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2-Butanona, suitable for HPLC, ≥99.7%

A7631

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(+)-Sodium L-ascorbate, crystalline, ≥98%

33209

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Ácido acético, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

11140

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(+)-Sodium L-ascorbate, BioXtra, ≥99.0% (NT)

205249

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Dicarbonato de di-terc-butilo, ReagentPlus^®, 99%

445460

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HATU, 97%

746452

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Sulfato de magnesio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

A4034

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(+)-Sodium L-ascorbate, powder, BioReagent, suitable for cell culture

M2643

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Sulfato de magnesio, BioReagent, suitable for cell culture, suitable for insect cell culture

746479

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Bromuro de litio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

71360

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Sodium carbonate decahydrate, puriss. p.a., ≥99.0% (T)

212865

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Copper(I) bromide, 98%

63136

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Sulfato de magnesio, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)

45726

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Ácido acético, for luminescence, BioUltra, ≥99.5% (GC)

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Magnesium sulfate solution, for molecular biology, 1.00 M±0.04 M

793612

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Sulfato de magnesio, anhydrous, free-flowing, Redi-Dri^™, reagent grade, ≥97%

1005706

USP

Ácido acético, United States Pharmacopeia (USP) Reference Standard

W200611

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Ácido acético, ≥99.5%, FCC, FG

110264

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2-Butanona, ReagentPlus^®, ≥99%

320129

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Acetyl chloride, reagent grade, 98%

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Tyramine, ≥98.0%