Saltar al contenido
Merck
  • A collaborative evaluation of the cytotoxicity of two surfactants by using the human corneal epithelial cell line and the WST-1 test.

A collaborative evaluation of the cytotoxicity of two surfactants by using the human corneal epithelial cell line and the WST-1 test.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics (2003-03-22)
Anne Huhtala, Päivi Alajuuma, Susi Burgalassi, Patrizia Chetoni, Horst Diehl, Maria Engelke, Marios Marselos, Daniela Monti, Periklis Pappas, M Fabrizio Saettone, Lotta Salminen, Marianthi Sotiropoulou, Hanna Tähti, Hannu Uusitalo, Michaela Zorn-Kruppa
RESUMEN

This study was undertaken to investigate the use of the in vitro test WST-1, an assay of cell proliferation and viability, for a preliminary safety evaluation of topical ophthalmic preparations. The cytotoxicity of two surfactants, benzalkonium chloride (BAC) and polyoxyethylene-20-stearyl ether (Brij78, PSE) was independently investigated in four laboratories in the EU by using an immortalized human corneal epithelial (HCE) cell line. The HCE cells were exposed to BAC and PSE for 5 min, 15 min, and 1 hour, and the results of the HCE-WST-1 tests were collected and compared. After one-hour exposure, the EC(50) values in BAC-treated cells in the presence of serum ranged between 0.0650 +/- 0.0284 (mean +/- SD) mM, and those in the absence of serum 0.0296 +/- 0.0081 mM. The corresponding values for PSE were 0.0581 +/-.0300 mM and 0.0228 +/-.0063 mM. There were variations in the results between different laboratories, with coefficients of variation ranging from 31 to 121%, mean 58%. The use of one-hour exposure time is to be preferred, and the elimination of serum in the culture medium is recommended to avoid both underestimation of toxic effects and variability of the test results.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Brij® S20
Sigma-Aldrich
Brij® S 100, average Mn ~4,670
Sigma-Aldrich
Brij® S10, average Mn ~711
Sigma-Aldrich
ECO Brij® S100, average Mn ~4,670
Sigma-Aldrich
ECO Brij® S20
Sigma-Aldrich
ECO Brij® S10, average Mn ~711