Saltar al contenido
Merck
  • Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria. I) Isolation and characterization of lactate dehydrogenases from thermophilic and mesophilic bacilli.

Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria. I) Isolation and characterization of lactate dehydrogenases from thermophilic and mesophilic bacilli.

Hoppe-Seyler's Zeitschrift fur physiologische Chemie (1979-07-01)
H P Schär, H Zuber
PMID114469
RESUMEN

Lactate dehydrogenases from thermophilic bacilli (Bacillus stearothermophilus, Bacillus caldotenax) and from mesophilic bacilli (Bacillus X1, Bacillus subtilis) have been isolated by a two-step purification procedure. Only one type (LDH-P4) composed of four identical subunits (Mr 34 000 or 36 000) was found in each bacillus. The tetrameric enzymes were characterized with respect to thermostability, pH and temperature dependence of the pyruvate reduction and the L-lactate oxidation, substrate specificity, saturation kinetics (Km values of pyruvate, lactate, NAD, NADH), pyruvate and oxamate inhibition, and activation by fructose bisphosphate. The thermophilic and mesophilic enzymes differ characteristically in these parameters. Preliminary structural data (amino acid composition, comparative N-terminal sequence analysis) show the expected close phylogenetic relationship (high degree of sequence homology), but also typical differences between thermophilic and mesophilic dehydrogenases, a suitable basis for further comparative studies.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from rabbit muscle, Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine heart, Type III, ammonium sulfate suspension, ≥500 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine heart, 1000 units/mL
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine heart, Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein
Sigma-Aldrich
Lactic Dehydrogenase, recombinant, ≥90 U/mg
Sigma-Aldrich
L-Lactic Dehydrogenase from porcine heart, ammonium sulfate suspension, ≥200 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine muscle, Type X, ammonium sulfate suspension, ≥600 units/mg protein