796-0657

Proteins, mixture

application for HPLC

Propiedades

• description: Separated on Discovery^® BIO PolyMA-WAX
• technique(s): HPLC: suitable
• test parameters: column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U); mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; detector: UV, 280 nm; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
• suitability: application for HPLC
• application(s): life science and biopharma

Descripción

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analito

Trypsin Inhibitor