Y2001

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan and uracil

• Sinónimos: Synthetic drop-out medium for yeast
• Número MDL: MFCD01095679
• Código UNSPSC: 12000000
• NACRES: ND.02

Propiedades

• Nivel de calidad: 100
• Formulario: powder
• aplicaciones: food and beverages

Descripción

Aplicación

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Componentes

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine, leucine, tryptophan and uracil. Each amino acid is present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)

Otras notas

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Cantidad

1.4 g will supplement 1 liter of medium.

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)