This kit is used only to quantify soluble dietary fibers.
TDF100A
Kit de análisis de la fibra alimentaria total
sufficient for ~100 assays
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1 KIT
US$ 679,99
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1 KIT
US$ 679,99
About This Item
Código UNSPSC:
12164500
NACRES:
NA.84
Productos recomendados
uso
sufficient for ~100 assays
Nivel de calidad
técnicas
photometry: suitable
aplicaciones
food and beverages
general analytical
temp. de almacenamiento
2-8°C
Aplicación
Este ensayo permite determinar el contenido de fibra alimentaria total de los alimentos utilizando una combinación de métodos enzimáticos y gravimétricos. Las muestras de alimentos secos y sin grasa se gelatinizan con α-amilasa termoestable y luego se someten a digestión enzimática con proteasas y amiloglucosidasas para eliminar las proteínas y el almidón presentes en la muestra. Se añade etanol para precipitar la fibra alimentaria soluble. A continuación se filtra el residuo y se lava con etanol y acetona. Después de secar, se pesa el residuo. La mitad de las muestras se utilizan para determinar las proteínas y el resto se convierten en ceniza. La fibra alimentaria total es el peso del residuo menos el peso de las proteínas y la ceniza.
Otras notas
Reactivos para la determinación de fibra alimentaria total.
Principio
Utiliza una combinación de métodos enzimáticos y gravimétricos. Este procedimiento se basa en el método publicado en los Métodos oficiales de análisis (Official Methods of Analysis), 15 edición, AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).
Solo componentes del kit
Referencia del producto
Descripción
- α-Amylase, Heat-stable 10 mL
- Amyloglucosidase 10 mL
- Celite 50 g
- Protease 500 mg
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
Órganos de actuación
Lungs, Respiratory system
Código de clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
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This Kit quantify solubles and insulubles fibers ?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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