SRP0706

KAT5 (TIP60), Active

recombinant

• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 25-35 nmol/min·mg (radiometric)
• mol wt: ~88 kDa
• concentration: 0.1 μg/μL
• accession no.: NM_006388
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... KAT5(10524)

Descripción

Application

Useful for acetyltransferase assay.

Biochem/physiol Actions

Full-length recombinant human KAT5 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. KAT5 or K (lysine) acetyltransferase 5 is a signaling protein that belongs to the MYST family of histone acetyl transferases (HATs). KAT5 was originally isolated as an HIV-1 TAT-interactive protein that plays an important role in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins ⁽¹⁾. KAT5 protein is a histone acetylase that is involved in DNA repair and apoptosis and plays an important role in signal transduction. KAT5 acts as a cofactor of TAT that is involved in the regulation of HIV gene expression. KAT5 is also a co-regulator of transcription factors that either promote or suppress tumorigenesis ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable