This control vector does not contain an shRNA insert.
SHC001
MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA
Contains no shRNA insert
Sinónimos:
MISSION® Control Vectors
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About This Item
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Nivel de calidad
Línea del producto
MISSION®
concentración
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Descripción general
The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones.[1][2] This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The pLKO.1-puro Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Aplicación
MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA has been used to synthesize and clone shRNAs (which target murine cluster of differentiation 36 (CD36), to generate lentiviral expression vectors.[3]
To see more application data, protocols, vector maps visit sigma.com/shrna.
Información legal
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
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Where is the hairpin inserted with Product SHC001, MISSION® pLKO.1-puro Control Vector?
1 answer-
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Does Product SHC001, MISSION® pLKO.1-puro Control Vector, convey puromycin resistance to transfected cells?
1 answer-
Yes, it has the puromycin resistance gene.
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Is Product SHC001, MISSION® pLKO.1-puro Control Vector, available as a glycerol stock?
1 answer-
The control vectors are available in plasmid DNA or the lentiviral transduction format.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the function of this negative control with Product SHC001, MISSION® pLKO.1-puro Control Vector?
1 answer-
It permits observation of cellular effects resulting from the transfection process. Cells transfected with the empty control provide a useful reference point for determining specific knockdown.
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