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Merck
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Key Documents

S500HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

500-HR, MW range 40-20,000 kDa (dextrans)

Sinónimos:

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Número de CAS:
65546-95-4
MDL number:
MFCD00165824
UNSPSC Code:
23151817
NACRES:
NA.56

product name

Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

40-20,000 kDa MW range (dextrans)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01

Application

Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A Lamberg et al.
The Journal of biological chemistry, 271(20), 11988-11995 (1996-05-17)
An efficient expression system for recombinant collagens would have numerous scientific and practical applications. Nevertheless, most recombinant systems are not suitable for this purpose, as they do not have sufficient amounts of prolyl 4-hydroxylase activity. Pro-alpha 1 chains of human
Takashi Ideue et al.
Genes & development, 21(16), 1993-1998 (2007-08-07)
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160.
Functional food applications of dextran from Weissella cibaria RBA12 from pummelo (Citrus maxima)
Baruah R, et al.
International Journal of Food Microbiology, 242, 124-131 (2017)
New structural features of Acacia tortuosa gum exudate
Martinez M, et al.
Food Chemistry, 182, 105-110 (2015)
Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive
Tingirikari JM, et al.
International Journal of Food Sciences and Nutrition, 65(6), 686-691 (2014)
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