R5403
Monoclonal Anti-Rhodopsin antibody produced in mouse
clone 1D4, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-CSNBAD1, Anti-OPN2, Anti-RP4
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About This Item
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
1D4, monoclonal
Formulario
buffered aqueous solution
reactividad de especies
human, rat, bovine
técnicas
immunocytochemistry: 1:1,000 using human retinal samples
western blot: 1:1,000 using Sf9 cells expressing the bovine gene
isotipo
IgG1
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... RHO(6010)
rat ... Rho(24717)
Descripción general
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin′s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
Especificidad
By Western blot, mouse monoclonal clone 1D4 anti-Rhodopsin antibody detects an ~40 kDa protein representing rhodopsin from Sf 9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope, and detects rhodopsin from human and bovine retinal samples.
Inmunógeno
bovine rhodopsin.
Aplicación
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Mouse monoclonal clone 1D4 anti-Rhodopsin antibody has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation. It is used as a probe to determine the presence and roles of rhodopsin in the conversion of light into electrochemical signals.
Forma física
Solution in phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Angelo Felline et al.
Computational and structural biotechnology journal, 19, 6020-6038 (2021-12-02)
Failure of a protein to achieve its functional structural state and normal cellular location contributes to the etiology and pathology of heritable human conformational diseases. The autosomal dominant form of retinitis pigmentosa (adRP) is an incurable blindness largely linked to
Silvia Ravera et al.
Cells, 12(17) (2023-09-08)
Dysfunction of the retinal pigment epithelium (RPE) is associated with several diseases characterized by retinal degeneration, such as diabetic retinopathy (DR). However, it has recently been proposed that outer retinal neurons also participate in the damage triggering. Therefore, we have
Jun Yin et al.
Investigative ophthalmology & visual science, 53(8), 4943-4951 (2012-06-30)
In experimental eye research, zebrafish has become a powerful model for human retina disorders. The purpose of the present study is the characterization of antibodies commonly employed in zebrafish models for rod photoreceptor degeneration. The 1D4 monoclonal antibody, developed against
Kirk Haltaufderhyde et al.
Photochemistry and photobiology, 91(1), 117-123 (2014-10-01)
Human skin is constantly exposed to solar light containing visible and ultraviolet radiation (UVR), a powerful skin carcinogen. UVR elicits cellular responses in epidermal cells via several mechanisms: direct absorption of short-wavelength UVR photons by DNA, oxidative damage caused by
Sandra Johnen et al.
BioMed research international, 2015, 863845-863845 (2015-12-24)
Pigment epithelium-derived factor (PEDF) is a potent multifunctional protein that inhibits angiogenesis and has neurogenic and neuroprotective properties. Since the wet form of age-related macular degeneration is characterized by choroidal neovascularization (CNV), PEDF would be an ideal candidate to inhibit
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