The Puralyzers are not designed for use with strong solvents. However, according to the chemical compatibility chart, Pur-A-Lyzer kits can be used with dimethylformamide (DMF), acetonitrile, and with less than 5% dimethyl sulfoxide (DMSO).
PURD10005
Pur-A-Lyzer™ Midi Dialysis Kit
Pur-A-Lyzer™ Midi Dialysis Kit
1 of 1 reviewers received a sample product or took part in a promotion
Midi 1000, capacity 50-800 μL, MWCO 1 kDa
Sinónimos:
Dialyzers, High through put dialysis tubing, puralyzer
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About This Item
Código UNSPSC:
41123100
NACRES:
NB.22
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Materiales
regenerated cellulose membrane
descripción
includes floating rack and handbook
envase
pkg of 5 Maxi dialysis tubes
capacidad
50-800 μL
tubo L × diám.
5.2 cm × 1.8 cm
tamaño de poro
1 kDa MWCO
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Categorías relacionadas
Descripción general
Pur-A-Lyzer dialysis tube kits are easy-to-use devices in a screw-cap tube format and are disposable. They contain a dual dialysis membrane window for buffer exchange. Samples are added and removed using a standard laboratory pipette and come in 4 different versions to accommodate a range of sample volumes.
- High sample recovery >97%
- Protease-, RNase-, and DNase-free
- Convenient sample loading- no syringes necessary
- Versatile- dialyze proteins, RNA, and DNA
The Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.
Aplicación
- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 μL
Midi: 50-800 μL
Maxi: 0.1-3 mL
Mega: 3-20 mL
Información legal
Pur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLC
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pH (low) insertion peptide (pHLIP) is a polypeptide from the third transmembrane helix of bacteriorhodopsin. The pH-dependent membrane insertion of pHLIP has been conveniently exploited for translocation of cargo molecules and as a novel imaging agent in cancer biology due
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Hi, can I use organic solvents with this kit? Specifically dimethylformamide (DMF). I have a 1500 Da peptide that I need to separate from a 600 Da byproduct. Neither are soluble in aqueous buffers.
1 answer-
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Does the Midi tube can be reuse for the same sample (example for the same protein or DNA target)?
1 answer-
This product is intended for single use. However, if the samples are identical in composition, reuse may be possible. Note that if the membrane is left to dry, repeat usage is not possible. Please see the link below to review the product Technical Bulletin:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/196/372/purd10005bul.pdfHelpful?
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What does the volume 50-800ul mean? why is the range so wide? Is the volume of the tube fixed? Does a kit equal to 5 such devices？
1 answer-
The Midi tube capacity is 50-800 microliters. This means that the sample volume should be in the range of 50-800 microliters. The volume of the tube is fixed. Each kit includes a floating rack along with the Pur-A-Lyzer tubes (PURNR-1EA). In the case of PURD10005, this includes 5 tubes with MWCO of 1 kDa.
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Can use for dialysis tube for MWCO 323-982 Da?
1 answer-
The product has a capacity of 50-800 μL and an MWCO of 1 kDa. Currently, there is no dialysis or centrifugal device available that would be appropriate for the specific range of 323-982 Da.
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