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Merck

PROTMEM

Millipore

ProteoPrep® Membrane Extraction Kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

temp. de almacenamiento

2-8°C

Nivel de calidad

Descripción general

ProteoPrep® Membrane Extraction Kit reagents utilize a powerful detergents for higher loading and resolution of proteins in 2D gels. Protein Extraction Reagent Types 3 and 4 are optimized for proteomic studies. Extraction reagents 3 and 4 are ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis.[1] This kit also includes reagents for the reduction and alkylation of disulfide bonds. The ProteoPrep® Membrane Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Aplicación

ProteoPrep® Membrane Extraction Kit is designed to prepare highly enriched membrane protein solutions from many types of cells. The final protein solution are suitable for 2D gel electrophoresis.[2]

Características y beneficios

  • Innovative detergent preparations - Improved solubility allows for higher protein loads and greater visibility of low abundance proteins in 2D gels.
  • Two pre-mixed solubilization solutions - Removes interfereing non-membrane proteins prior to extractions, resulting in uncluttered 2D arrays.
  • Pre-measured reducing & alkylating reagents - Easy-to-use reagents provide improved IEF resolution.

Información legal

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS

  • C0356Protein Extraction Reagent Type 4 23 mLSDS

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto de inflamabilidad (°F)

186.8 °F - closed cup

Punto de inflamabilidad (°C)

86 °C - closed cup


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Sreepriya Nair et al.
Electrophoresis, 32(24), 3621-3629 (2011-11-22)
Performing 2-DE of lipid-rich multilamellar membranes like myelin is a cumbersome task. However, for understanding its molecular organization and changes during diseases, identification of proteins of myelin is essential. Although the 2-D-proteomic approach of myelin has been employed to understand
Lili Xu et al.
The Journal of general virology, 90(Pt 11), 2695-2703 (2009-07-25)
Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has
Carla Cacciotto et al.
BMC microbiology, 10, 225-225 (2010-08-27)
Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with the loss of many biosynthetic pathways and of the cell wall. In this work, the liposoluble protein complement of Mycoplasma agalactiae, a minimal bacterial
Fang Chen et al.
Proteomics, 7(9), 1529-1539 (2007-04-05)
Plant plasma membrane (PM) proteins play important roles in signal transduction during defense response to an attacking pathogen. By using an improved method of PM protein preparation and PM-bound green fluorescent protein fusion protein as a visible marker, we conducted
Medicharla Venkata Jagannadham et al.
Journal of proteomics, 75(8), 2488-2499 (2012-03-16)
Antarctic bacteria are adapted to the extremely low temperature. The transcriptional and translational machineries of these bacteria are adapted to the sub-zero degrees of temperature. Studies directed towards identifying the changes in the protein profiles during changes in the growth

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