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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Sinónimos:

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.54
Formulario:
DMSO solution
temp. de almacenamiento:
−20°C
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Protease and Phosphatase Inhibitor Cocktail,

Formulario

DMSO solution

Nivel de calidad

200

envase

pkg of 1 ml, 5 ml ×

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Descripción general

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Aplicación

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction[1]
  • to lyse purified CD4+ T cells[2]
  • to prepare protein extracts from placental tissues and cells[3]

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.[4][5]

Nota de preparación

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H315,H319

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
0000201980
0000359849
0000359848
0000359849
0000359848

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Questions

1–2 of 2 Questions  

  1. Does PPC1010 contain EDTA?

    1 answer

    1. PPC1010 does not contain EDTA

      Helpful?

  2. Does these Protease and Phosphatase Inhibitor Cocktail contains any type of metal chelator or EDTA?

    1 answer

    1. This product does not contains EDTA or other chelators. See the link below to review the product datasheet which includes the cocktail components:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/180/ppc1010dat.pdf

      Helpful?

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