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PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Sinónimos:

Protease Assay Kit

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.26

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Aplicación

Fluorescent Protease Detection Kit Video Protocol
Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Idoneidad

Detection limit: approx. 50 ng trypsin

Cantidad

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Nota de análisis

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Solo componentes del kit

Referencia del producto
Descripción
  • Incubation buffer 25 mL
  • FITC-casein solution 5 mL
  • Assay buffer 200 mL
  • Protease standard (trypsin) 20 μg
  • FITC control 50 mg
  • Trichloroacetic acid (TCA) solution 30 mL

Palabra de señalización

Danger

Frases de peligro

H314,H317,H334,H335,H410

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000401713
0000401713
SLCM8764
SLCM8767
SLCL1456

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Visite la Librería de documentos

Céline Adam et al.
PloS one, 7(5), e38138-e38138 (2012-06-14)
Mitochondria have their own ATP-dependent proteases that maintain the functional state of the organelle. All multicellular eukaryotes, including filamentous fungi, possess the same set of mitochondrial proteases, unlike in unicellular yeasts, where ClpXP, one of the two matricial proteases, is
Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles.
Sharma A, et al.
IEEE Transactions on Magnetics, 43, 2418-2420 (2007)
Veronica Tjomsland et al.
European journal of immunology, 43(6), 1470-1483 (2013-03-26)
Induction of optimal HIV-1-specific T-cell responses, which can contribute to controlling viral infection in vivo, depends on antigen processing and presentation processes occurring in DCs. Opsonization can influence the routing of antigen processing and pathways used for presentation. We studied
Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes
Kieloaho AJ, et al.
Soil Biology and Biochemistry, 96(10), 97-106 (2016)
Joseph S Rom et al.
Virulence, 12(1), 584-600 (2021-02-05)
Using the USA300, methicillin-resistant Staphylococcus aureus strain LAC, we previously examined the impact of regulatory mutations implicated in biofilm formation on protease production and virulence in a murine sepsis model. Here we examined the impact of these mutations in the
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Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Protocolos

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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