PCRISPR004
Mouse CRISPR Brie Knockout Library
Sinónimos:
CRISPR Brie Library, CRISPR Library for Mice, Mouse Knockout Library
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About This Item
UNSPSC Code:
41105904
NACRES:
NE.02
Productos recomendados
packaging
pkg of 5 vials (5x200µL aliquots )
concentration
≥5x108 VP/ml (via p24 Assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Application
- Functional Genomics/Target Validation
- Unbiased wholed genome forward genetic screening
- Validated positive and negative controls
- Set up and optimization of screen assay
Features and Benefits
- Focus on your research, and we will generate your lentivirus screening library.
- Use CRISPR nucleases to knockout protein-coding genes to assess their function.
- Mouse Genes targeted 19,674
- Compact library of ~ four gRNAs per gene (78,637 total)
- Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9.
Principle
In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
John G Doench et al.
Nature biotechnology, 34(2), 184-191 (2016-01-19)
CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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