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Merck
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Key Documents

P3369

Sigma-Aldrich

Protamine sulfate

solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction

Sinónimos:

Salmine sulfate from salmon sperm, Protamine sulfate

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About This Item

MDL number:
MFCD00132091
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Protamine sulfate, meets USP testing specifications

agency

meets USP testing specifications

assay

≥92% (chromatography)
90.0-110.0% dry basis

form

solid

mol wt

Mr 5000-10000

technique(s)

transduction: suitable

shipped in

ambient

storage temp.

2-8°C

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General description

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

Application

Protamine sulfate has been used in the transduction of
  • lentiviruses into human embryonic 293T cells
  • shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
  • lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells

Biochem/physiol Actions

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Moosung Lee et al.
eLife, 9 (2020-12-18)
The immunological synapse (IS) is a cell-cell junction between a T cell and a professional antigen-presenting cell. Since the IS formation is a critical step for the initiation of an antigen-specific immune response, various live-cell imaging techniques, most of which
Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation
Ainle FN, et al.
Blood, 114(8), 1658-1665 (2009)
Meagan R Rollins et al.
Current protocols in immunology, 129(1), e97-e97 (2020-05-21)
T lymphocytes are capable of specific recognition and elimination of target cells. Physiological antigen recognition is mediated by the T cell receptor (TCR), which is an alpha beta heterodimer comprising the products of randomly rearranged V, D, and J genes.
Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs
Hematopoietic Differentiation of Human Pluripotent Stem Cells, 91-101 (2015)
F L Sorgi et al.
Gene therapy, 4(9), 961-968 (1998-02-12)
A polycationic peptide, protamine sulfate, USP, has been shown to be able to condense plasmid DNA efficiently for delivery into several different types of cells in vitro by several different types of cationic liposomes. The monovalent cationic liposomal formulations (DC-Chol
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