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Merck

N3254

Sigma-Aldrich

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

Sinónimos:

4-Nitrophenyl phosphate bis(tris) salt

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About This Item

Fórmula lineal:
(C6 H4 N O6 P)( C4 H12 N O3)2
Número de CAS:
Peso molecular:
461.36
Beilstein:
1578142
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
NACRES:
NA.83

Nombre del producto

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Nivel de calidad

Ensayo

≥97% (enzymatic)

Formulario

powder

solubilidad

water: 50 mg/mL, clear, colorless to light yellow

temp. de almacenamiento

−20°C

cadena SMILES

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

Clave InChI

XXAXKCWOTRABOW-UHFFFAOYSA-N

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Aplicación

4-Nitrophenyl phosphate di(tris) salt has been used:
  • as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
  • to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
  • for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Acciones bioquímicas o fisiológicas

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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