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MP0040A

Sigma-Aldrich

LookOut® Mycoplasma qPCR Detection Kit

Sinónimos:

Quantiative PCR mycoplasma detection kit

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About This Item

Número CE:
200-664-3
Código UNSPSC:
12352207
NACRES:
NA.75

uso

sufficient for 25 reactions

Nivel de calidad

200

calidad

Not suitable for clinical diagnostic use.

envase

pkg of 1 kit

condiciones de almacenamiento

dry at room temperature

técnicas

PCR: suitable

aplicaciones

detection
microbiology

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

Aplicación

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Información legal

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
0000353083
0000308819
0000353083
SLCR5129
SLCR0581

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Ver todo

Protocolos

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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