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M9400

Sigma-Aldrich

β-Mannosidase from Helix pomatia

5-30 units/mL, ammonium sulfate suspension, crude extract

Sinónimos:

β-D-Mannoside mannohydrolase

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About This Item

Número de CAS:
9025-43-8
Comisión internacional de enzimas:
3.2.1.25 (BRENDA, IUBMB)
Número MDL:
MFCD00130638
Código UNSPSC:
12352204
NACRES:
NA.32

Formulario

ammonium sulfate suspension

Nivel de calidad

200

calidad

crude extract

mol peso

94 kDa

concentración

5-30 units/mL

actividad extraña

β-N-acetylglucosaminidase and α-mannosidase <1.0%

temp. de almacenamiento

2-8°C

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Descripción general

β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.

Aplicación

β-Mannosidase from Helix pomatia has been used:
  • in the digestion of glycans for enzymatic analysis
  • in the treatment of labeled monogalactosyl Manno-oligosaccharides
  • in non-mammalian glycosidase inhibition assays

Acciones bioquímicas o fisiológicas

β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.

Definición de unidad

One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.

Forma física

Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0

Inhibidor

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000367558
0000367558
0000295508
SLCF8311
SLBW8901

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María del Carmen Rodríguez-Gacio et al.
Journal of experimental botany, 63(11), 3976-3988 (2012-05-04)
The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the
Jia Zheng et al.
Bioresource technology, 118, 257-264 (2012-06-19)
An industrial medium, Corn Steep Liquor Powder Dextrose (CSD medium) was developed for constitutive expression of recombinant β-mananase by Pichia pastoris. The β-mananase activity (513 U/mL) with CSD medium was 1.64- and 2.5-fold higher than with YPD and BSM in
A P Félix et al.
Journal of animal science, 90(9), 3060-3067 (2012-05-16)
This experiment aimed at evaluating the effects of including the enzyme, β-mannanase, in dog (Canis lupus familiaris) diets based on either poultry (Gallus gallus domesticus) by-product meal (PBM) or soybean [Glycine max (L.) Merr.] Meal (SBM). The second objective was
Guojie Jin et al.
Bioresource technology, 111, 378-382 (2012-03-01)
Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of
Yuya Kumagai et al.
Biochimie, 94(12), 2783-2790 (2012-09-27)
Mannanase is an important enzyme involved in the degradation of mannan, production of bioactive oligosaccharides, and biobleaching of kraft pulp. Mannanase must be thermostable for use in industrial applications. In a previous study, we found that the thermal stability of
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