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K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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About This Item

UNSPSC Code:
12161702
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

pH

6.1-6.7

solubility

water: 9.5 g/L, clear

storage temp.

2-8°C

General description

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Application

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 1.26 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Activation of G Protein-Coupled Receptor 43 in Adipocytes Leads to Inhibition of Lipolysis and Suppression of Plasma Free Fatty Acids
Hongfei G, et al.
Endocrinology, 149, 4519?4526-4519?4526 (2008)
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European journal of immunology, 38(12), 3499-3507 (2008-11-11)
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes
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Nature communications, 12(1), 3340-3340 (2021-06-09)
Hepatic gluconeogenesis is essential for glucose homeostasis and also a therapeutic target for type 2 diabetes, but its mechanism is incompletely understood. Here, we report that Sam68, an RNA-binding adaptor protein and Src kinase substrate, is a novel regulator of
The Hypothalamic-Pituitary-Adrenal Axis Response to Stress in Mice Lacking Functional Vasopressin V1b Receptors
Stephen J L, et al.
Endocrinology, 148, 849?856-849?856 (2007)
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Journal of molecular and cellular cardiology, 92, 174-184 (2016-02-09)
One approach to improve contraction in the failing heart is the administration of calcium (Ca(2+)) sensitizers. Although it is known that levosimendan and other sensitizers bind to troponin C (cTnC), their in vivo mechanism is not fully understood. Based on
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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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