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Merck

I4401

Sigma-Aldrich

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Sinónimos:

IFN-αA/D

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

human

Nivel de calidad

recombinante

expressed in E. coli

Ensayo

≥95%

Formulario

buffered aqueous solution

mol peso

19 kDa

envase

pkg of 100K units

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

color

colorless

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... IFNA2(3440)

Aplicación

Interferon-αA/D human has been used:
  • for identification of coronavirus replicase inhibitors
  • in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication
  • in combination with vitamin D to check reduction in HCV protein production in cell culture

Acciones bioquímicas o fisiológicas

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.
This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Forma física

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Nota de análisis

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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Journal of virology, 89(13), 6711-6724 (2015-04-17)
High-grade tumors in the brain are among the deadliest of cancers. Here, we took a promising oncolytic virus, vesicular stomatitis virus (VSV), and tested the hypothesis that the neurotoxicity associated with the virus could be eliminated without blocking its oncolytic
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Journal of virology, 96(11), e0036422-e0036422 (2022-05-20)
Effective broad-spectrum antivirals are critical to prevent and control emerging human coronavirus (hCoV) infections. Despite considerable progress made toward identifying and evaluating several synthetic broad-spectrum antivirals against hCoV infections, a narrow therapeutic window has limited their success. Enhancing the endogenous
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Journal of virology & antiviral research, 3(3), doi:10-doi:10 (2015-11-26)
Previous studies suggest that low serum 25-hydroxyvitamin D [25(OH) D] levels are associated with reduced responsiveness to interferon and ribavirin therapy. We investigated the impact of vitamin D metabolites on HCV and cellular gene expression in cultured hepatoma cells. HCV

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