GE17-5131-01

GSTrap^™ Fast Flow

Cytiva 17-5131-01, pack of 1 × 5 mL

• Código UNSPSC: 23151817
• NACRES: NA.25

Propiedades

• ligand: glutathione and 10-carbon linker arm
• envase: pack of 1 × 5 mL
• fabricante / nombre comercial: Cytiva 17-5131-01
• condiciones de almacenamiento: (20% Ethanol)
• Parámetros: <20 mL/min flow rate; 42 psi
• lecho tamaño: 16 mm × 25 mm
• lecho volumen: 5 mL
• columna D.I.: 16 mm
• Matriz: highly cross-linked 4% agarose
• diámetro promedio: 90 μm
• cleaning: 3-12
• Intervalo de trabajo: 3-12
• capacidad: >10 mg/mL binding capacity
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Aplicación

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Características y beneficios

• Fast and simple one-step purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast FLow provides good fLow properties.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids