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Merck

FD10S

Sigma-Aldrich

Isotiocianato de fluoresceína-dextrano

average mol wt 10,000

Sinónimos:

FITC–Dextrano

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

sugar beets root

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 10,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to yellow-orange

solubility

water: 25 mg/mL, cloudy to hazy, yellow to orange

storage temp.

2-8°C

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Application

Dextrano marcado con isotiocianato de fluoresceína para su posible uso en estudios de perfusión en animales.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Antonini, E. et al.
Biopolymers null
Clotilde Cadart et al.
Nature communications, 9(1), 3275-3275 (2018-08-18)
Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on various mammalian cell
The structure of NRRL B-512F dextran, methylation studies.
Van Cleve JW, Schacfer WC and Rist CE
Journal of the American Chemical Society, 78(17), 4435-4438 (1956)
Evaluation of the Periodate Oxidation Method for Structural Analysis of Dextrans.
John C. Rankin , Allene Jeanes
Journal of the American Chemical Society, 17, 4435-4441 (1954)
Anna Marchetti et al.
BMC research notes, 2, 7-7 (2009-01-14)
Dictyostelium amoebae are frequently used to study the organization and function of the endocytic pathway, and specific protocols are essential to measure the dynamics of endocytic compartments and their internal pH. We have revisited these classical protocols to measure more

Contenido relacionado

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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