We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).
F7378
Fluorescein diacetate
Powder
Sinónimos:
3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
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About This Item
Fórmula empírica (notación de Hill):
C24H16O7
Número de CAS:
Peso molecular:
416.38
Beilstein:
62575
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nombre del producto
Fluorescein diacetate, used as cell viability stain
Nivel de calidad
Formulario
powder
técnicas
titration: suitable
color
white to faint yellow
mp
200-203 °C (lit.)
solubilidad
acetone: 25 mg/mL
ε (coeficiente de extinción)
≥86000 at 488-494 nm in 0.1 M NaOH
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
room temp
cadena SMILES
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
Clave InChI
CHADEQDQBURGHL-UHFFFAOYSA-N
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Categorías relacionadas
Descripción general
Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.[1]
Aplicación
Sustratos
Lipase substrate
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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What solubility information do you have on Product F7378, Fluorescein diacetate?
1 answer-
Helpful?
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After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?
1 answer-
The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.
Helpful?
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Is Product F7378, Fluorescein diacetate, a fluorescent compound?
1 answer-
No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein. Only the fluorescein form is fluorescent.
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How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?
1 answer-
Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What are some common applications for Product F7378, Fluorescein diacetate?
1 answer-
This compound is used extensively for the staining of living cells. It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes, Anal. Chem. 36, 409, 1964."" "
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