This product has not been tested for any specific application, including in vivo use. Suitability would have to be determined experimentally by the end user. Historical information suggests that a concentration of 0.5% Evans Blue in PBS at pH 7.4 has been used successfully in staining tissue sections. This concentration may also be effective starting point for in vivo applications. Please refer to available literature for additional information.
E2129
Evans Blue
Dye content ≥75 %, Powder
Sinónimos:
Direct Blue 53
About This Item
Productos recomendados
Nombre del producto
Evans Blue, Dye content ≥75 %
Formulario
powder
Nivel de calidad
composición
Dye content, ≥75%
técnicas
titration: suitable
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
room temp
cadena SMILES
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
Clave InChI
ATNOAWAQFYGAOY-GPTZEZBUSA-J
Información sobre el gen
human ... PTPN1(5770) , PTPRF(5792)
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Categorías relacionadas
Descripción general
Aplicación
Acciones bioquímicas o fisiológicas
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Carc. 1B
Código de clase de almacenamiento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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What amount and concentration should I use Evans-Blaun injecting it into the ear skin of a mouse to see Evans-Blue in the draining Lymphnodes afterwards?
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How to prepare a stock 1% solution using E2129, and is ther any guidance on its use?
1 answer-
A 0.5% solution of Evans Blue in PBS, pH 7.4. Product P3813 should be used as the PBS, which will serve as the stock solution. For use, 3-4 drops should be added to 100 mL of PBS P3813. This should be used as the final rinse with a 5-minute wash. After rinsing briefly, coverslip the sample.
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What is the stability of the E2129 solution once prepared?
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The stability of the solution will depend on whether the solutions are sterile filtered or contaminated through routine use. It's recommended to store the solutions in the dark at 2-8°C.
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Should Evans Blue solutions for fluorescent assays be aged or cured for 7 days before use?
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While some dyes require aging before use, there is no indication that Evans Blue solutions need to be aged or cured before use.
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How do I dilute 80% purity evans blue powder into a 2% solution with normal saline?
1 answer-
This product is soluble in water at 60 mg/mL. For typical laboratory staining applications, a 1X PBS solution is also commonly used. Solubility in normal saline has not been evaluated, but rates are expected to be similar. A 2% solution would be 20 mg/mL. The dye content is lot-specific and reported in the Certificate of Analysis. Please see the link below to access a sample or batch-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e2129#product-documentationHelpful?
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What is the solution stability of Product E2129, Evans Blue?
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This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product E2129, Evans Blue, be dissolved?
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This product is soluble in water, alcohol, acids and alkalies.
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Can Evans Blue, Product E2129, be used as a counterstain for FITC?
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This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).
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What is the shelf life of Product E2129, Evans Blue?
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This product has a retest date of 4 years. This information can be found on the Certificate of Analysis.
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