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D8900

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.71

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

also commonly purchased with this product

Referencia del producto
Descripción
Precios

supplement

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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