The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate. Historical data also note that this material may be reconstituted in PBS or other physiologic buffers containing Ca2+, culture medium, or water; however, no concentration is provided.
D4818
Dispase I
protease
Sinónimos:
Proteasa from Bacillus polymyxa
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nivel de calidad
Formulario
lyophilized solid
actividad específica
≥10 unit/mg solid
técnicas
cell culture | mammalian: suitable
single cell analysis: suitable
temp. de almacenamiento
2-8°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Aplicación
Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.
Dispase I has been used in lung digestion and processing for flow staining, as well as for CD4 cell isolation in mice.[1] The enzyme has also been used to digest excised wounds and a small amount of surrounding skin for the detection of GFP+ (green fluorescence protein) cells. This study to investigated the effect of differentiation and angiogenesis of bone marrow-derived mesenchymal stem cells on wound healing.[2] It has also been used to remove the epidermis during the isolation of dermal fibroblasts from mice.[3]
Suitable for use in preparation of single cell suspension for sequencing.
Suitable for use in preparation of single cell suspension for sequencing.
Acciones bioquímicas o fisiológicas
Dispase I is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. It can also separate intact epithelial sheets in culture from the substratum. The enzyme preserves the viability of the epithelial cells while cleaving the basement membrane zone region. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] It hydrolyzes N-terminal peptide bonds of non-polar amino acid residues. It preferentially attacks denatured and intercellular proteins with exposed hydrophobic amino acid residues. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[5] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.
Definición de unidad
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Forma física
lyophilized powder containing calcium acetate
Información legal
Dispase is a registered trademark of Godo Shusei Co., Ltd.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Alizée Vercauteren Drubbel et al.
Cell stem cell, 28(8), 1411-1427 (2021-04-22)
Columnar metaplasia of the esophagus is the main risk factor for esophageal adenocarcinoma. There is a lack of evidence to demonstrate that esophageal progenitors can be the source of columnar metaplasia. In this study, using transgenic mouse models, lineage tracing
Alessandra Sacco et al.
Nature, 456(7221), 502-506 (2008-09-23)
Adult muscle satellite cells have a principal role in postnatal skeletal muscle growth and regeneration. Satellite cells reside as quiescent cells underneath the basal lamina that surrounds muscle fibres and respond to damage by giving rise to transient amplifying cells
Yaojiong Wu et al.
Stem cells (Dayton, Ohio), 25(10), 2648-2659 (2007-07-07)
Although chronic wounds are common, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefit of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing. Using an excisional wound splinting model, we showed
Liwen Chen et al.
PloS one, 4(9), e7119-e7119 (2009-09-23)
Studies have shown that allogeneic (allo-) bone marrow derived mesenchymal stem cells (BM-MSCs) may enhance tissue repair/regeneration. However, recent studies suggest that immune rejection may occur to allo-MSCs leading to reduced engraftment. In this study, we compared allo-BM-MSCs with syngeneic
Marco Tatullo et al.
Materials (Basel, Switzerland), 12(4) (2019-02-20)
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting
Artículos
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
Contenido relacionado
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
-
How to disolve dispase? Can I use mediums as DMEM, PBS ...?
1 answer-
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the solubility of Product D4818, Dispase I?
1 answer-
The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate.
Helpful?
-
-
What is the difference between Product D4693, Dispase II and Product D4818, Dispase I?
1 answer-
Product D4693 is a lyophilized powder containing calcium acetate and milk sugar as a stabilizer. The activity specification is >0.5 unit/mg solid. Product D4818 does not contain any milk sugar and has an activity specification of >10 units/mg solid.
Helpful?
-
-
What is the activity of the Product D4818, Dispase, which I received?
1 answer-
Each lot will have a specific activity that can be found on our website on the certificate of analysis.
Helpful?
-
-
At what concentration should Product D4818, Dispase, be used?
1 answer-
To dissociate cells from ECM (extracellular matrix) use at a concentration of 0.6 - 2.4 units/ml.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
