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Key Documents

CLS356234

Corning® Matrigel® Basement Membrane Matrix

LDEV-Free, pack of 5 mL

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About This Item

UNSPSC Code:
41116155
NACRES:
NA.7

feature

LDEV-Free

packaging

pack of 5 mL

manufacturer/tradename

Corning 356234

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General description

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
Tested and found negative for bacteria, fungi, and mycoplasma
Protein concentrations are determined by Lowry method
Endotoxin units are measured by Limulus Amoebocyte Lysate assay
Corning Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
Source
Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application

Cell Growth and Differentiation
Metabolism/Toxicology Studies
Invasion Assays
in vitro and in vivo Angiogenesis Assays
in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

For Customers in California only: WARNING Please refer to Safety Data Sheet (SDS) Section 15 or Letter in Lieu for more information regarding California Proposition 65.

Legal Information

Corning is a registered trademark of Corning, Inc.
Matrigel is a registered trademark of Corning, Inc.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Protocolos

Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Learn how the Corning® Matrigel® Basement Membrane Matrix can be used to boost your 3D Cell Culture activities.

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

Learn the results that come from changing the protein concentration of Corning® Matrigel®.

Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Learn how the Corning® Matrigel® Basement Membrane Matrix can be used to boost your 3D Cell Culture activities.

Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Learn how the Corning® Matrigel® Basement Membrane Matrix can be used to boost your 3D Cell Culture activities.

Ver todo

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