BB

87101201, fish trunk, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: BB, 87101201
• biological source: fish trunk
• growth mode: Adherent
• karyotype: 2n = 60
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Fish Brown Bullhead posterior trunk tissue

Cell Line Description

Derived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.

Application

Virus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virus

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. Note: The cells do not grow well on glass. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for a

Other Notes

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