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C5338

Sigma-Aldrich

Cyanogen bromide-activated-Sepharose™ 4 Fast Flow

lyophilized powder

Sinónimos:

Cyanogen bromide-activated Agarose

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About This Item

Número de CAS:
68987-32-6
Número MDL:
MFCD00163385
Código UNSPSC:
41106500
NACRES:
NA.56

Formulario

lyophilized powder

Nivel de calidad

200

técnicas

affinity chromatography: suitable

Matriz

Sepharose 4 Fast Flow

grupo activo de la matriz

cyanate or related structures

espaciador de matriz

1 atom (when ligands are coupled via isourea derivative or related linkage)

hinchazón

1 g swells to 4-5 mL gel

tamaño de partícula

45—165 μm

capacidad

6-11 μmol/mL coupling capacity (6-AKS)

temp. de almacenamiento

2-8°C

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Descripción general

C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated Sepharose™ 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

Aplicación

Cyanogen bromide-activated-Sepharose™ is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose™ has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Forma física

Lyophilized powder with sugar stabilizers

Información legal

Sepharose is a trademark of Cytiva

sustituido por

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000288582
SLCL9403
SLCH8520
SLCC5852
SLBW7796

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Joyce M Bevington et al.
Virology, 357(1), 102-113 (2006-09-09)
Adeno-associated virus (AAV) is a human parvovirus that normally requires a helper virus such as adenovirus (Ad) for replication. The four replication proteins (Rep78, 68, 52 and 40) encoded by AAV are pleiotropic effectors of virus integration, replication, transcription and
F Jafri et al.
Biotechnology and bioengineering, 56(6), 605-609 (2008-07-22)
Polyclonal antibodies directed against the yeast invertase glycosyls were raised by immunizing rabbits with neoglycoprotein-I and neoglycoprotein-II. The neoglycoproteins were prepared by separately coupling the N-linked large and small molecular weight yeast invertase oligosaccharides respectively to bovine serum albumin with
M H Zeng et al.
Transplantation proceedings, 38(10), 3312-3314 (2006-12-19)
Human natural killer (NK) cells, which can mediate direct lysis of porcine endothelial cells, play an important role in xenograft rejection. HLA-G, which is a critical molecule in maintaining maternal immune tolerance of semi-allogenic fetus, is able to protect susceptible
Regina Maria Sousa de Araújo et al.
Plant science : an international journal of experimental plant biology, 183, 20-26 (2011-12-27)
Hemagglutinating activity has been associated to presence of lectin, carbohydrate-binding proteins. In this work Crataeva tapia bark lectin (CrataBL) was purified in milligram quantities (28 mg per g of bark) by ion exchange chromatography. The lectin was thermo-stable, ion-independent and

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