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Documentos clave

C-39891

Sigma-Aldrich

Hematopoietic Progenitor Expansion Medium DXF

Cytokine Mix E for 500 ml

Sinónimos:

Primary Cell Culture Media

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

Formulario

aqueous solution

envase

pkg of 1 vial

técnicas

cell culture | mammalian: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Cytokine Mix E for HPC-Expansion Medium DXF

Aplicación

Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium DXF (C-28021). The animal component-free, defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The optimized formulation allows vigorous expansion of hematopoietic progenitor cells, while maintaining their primitive phenotype. Differentiation is largely inhibited.

Calidad

All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Almacenamiento y estabilidad

Store the Cytokine Mix at -20°C in the dark immediately after arrival. If stored properly, the it is stable until the expiry date stated on the label. Medium supplemented with Cytokine Mix E should be used within 2 weeks after addition of the cytokines. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rutina de subcultivo

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages. 

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

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Protocolos

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

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