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Merck
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AB0300

Sigma-Aldrich

Alkaline Phosphatase Blue Membrane Substrate Solution

alkaline phosphatase substrate, solution

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About This Item

Código UNSPSC:
12352204
eCl@ss:
42010105
NACRES:
NA.83
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate

Formulario

solution

Nivel de calidad

200

uso

sufficient for 400 mL working substrate

temp. de almacenamiento

2-8°C

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Descripción general

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family.[1] It is present in bile ducts, bone, intestine, placenta and tumors.[2]

Aplicación

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
  • to analyse direct alkaline phosphatase (AP) activity[3]
  • to perform alkaline phosphatase assays with colorimetric system[4]
  • to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3[5][6]

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

Forma física

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Información legal

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCM0073
SLCL7179
SLCF1959
SLCB1691
SLBZ1825

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Visite la Librería de documentos

Inhibition of alkaline phosphatase: an emerging new drug target
Al-Rashida M and Iqbal J
Mini-Reviews in Medicinal Chemistry, 15(1), 41-51 (2015)
The Short Isoform of NF-YA Belongs to the Embryonic Stem Cell Transcription Factor Circuitry
Dolfini D, et al.
Stem Cells, 30(11), 2450-2459 (2012)
Generation of a human iPSC line from a patient with Leigh syndrome
Galera T, et al.
Stem Cell Research, 16(1), 63-66 (2016)
Giulia Coppiello et al.
Stem cell research, 21, 47-50 (2017-07-06)
We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells
Jaundice
Nelson Pediatric Symptom-Based Diagnosis, 255-274 (2018)
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Artículos

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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