Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

A9810

Sigma-Aldrich

Fragmento 1-42 de la proteína amiloide β

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Fórmula empírica (notación de Hill):
C203H311N55O60S
Número de CAS:
107761-42-2
Peso molecular:
4514.04
MDL number:
MFCD00163049
UNSPSC Code:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

assay

≥95% (HPLC)

Quality Level

200

form

powder

UniProt accession no.

P05067

storage temp.

−20°C

SMILES string

S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(

InChI

1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112

InChI key

DZHSAHHDTRWUTF-SIQRNXPUSA-N

Gene Information

human ... APP(351)

Amino Acid Sequence

Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

General description

La proteína β amiloide se produce a partir de la proteína precursora amiloide-β (APP). Consta de dos variantes C terminales, como la Aβ 1-42 de cola larga y la Aβ 1-40 de cola corta.[1] La APP está localizada en el cromosoma humano 21q21.3.[2]

Application

El fragmento 1-42 de la proteína amiloide β se ha utilizado:
  • para la preparación del oligómero Aβ 1-42[3][4]
  • para análisis mediante inmunotransferencia western[5]
  • para la prueba de interferencia del ensayo de la Aβ42 plasmática mediante reducción inmunomagnética (IMR)[6]
  • para estudiar los efectos del resveratrol sobre el deterioro inducido por la Aβ 1-42 en el aprendizaje espacial, la memoria y la plasticidad sináptica[7]
  • para investigar el efecto de la Aβ en los cultivos de células epiteliales[1]

Biochem/physiol Actions

El fragmento 1-42 de la proteína β amiloide (Aβ 1-42) tiene propiedades antioxidantes y neuroprotectoras.[8] La acumulación de la proteína β amiloide se asocia con la enfermedad de Alzheimer (AD) y el síndrome de Down.[9] La Aβ 1-42 regula el transporte del colesterol[10] y puede actuar como un factor de transcripción.[11] Puede que posea propiedades anti-inflamatorias y antimicrobianas.[12]
La fragmento predominante de la proteína β amiloide encontrado en los pacientes con enfermedad de Alzheimer y síndrome de Down.

related product

Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000388520
0000387190
0000368432
0000336675
0000331858

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Resveratrol ameliorates spatial learning memory impairment induced by Abeta1-42 in rats
Wang R, et al.
Neuroscience, 344, 39-47 (2017)
Kyunghee Byun et al.
PloS one, 7(5), e37917-e37917 (2012-06-05)
Advanced glycation end products (AGEs) have long been considered as potent molecules promoting neuronal cell death and contributing to neurodegenerative disorders such as Alzheimer's disease (AD). In this study, we demonstrate that AGE-albumin, the most abundant AGE product in human
Function of beta-amyloid in cholesterol transport: a lead to neurotoxicity
Yao ZX and Papadopoulos V
Faseb Journal, 16(12), 1677-1679 (2002)
Alzheimer's disease and the amyloid-beta peptide
Murphy MP and LeVine III H
Journal of Alzheimer'S Disease, 19(1), 311-323 (2010)
Plasma Amyloid-beta(Abeta 42) Correlates with Cerebrospinal Fluid Abeta 42 in Alzheimer?s Disease
Teunissen CE, et al.
Journal of Alzheimer'S Disease, 1-7 (2018)
Ver todos los artículos relacionados

Questions

1–10 of 10 Questions  

  1. After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?

    1 answer

    1. This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  2. What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?

    1 answer

    1. These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
      PP69 Calbiochem:
      https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
      A9810 Sigma-Aldrich:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  3. It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution

    1 answer

    1. This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.

      Helpful?

  4. How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?

    1 answer

    1. It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.

      Helpful?

  5. in which solvent it should be dissolved ?

    1 answer

    1. As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.

      See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf

      Helpful?

  6. Is the mouse protein fragment the same as the human protein fragment in Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The homology is 93% by identity, 95% positives...  no gaps

      Helpful?

  7. What is the source of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. This protein fragment is a synthetic organic.  The gene for this peptide is located on human chromosome 21.

      Helpful?

  8. What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992

      Helpful?

  9. What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?

    1 answer

    1. The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico