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Merck

803057

Sigma-Aldrich

Manganese Peroxidase from white-rot fungus (Phanerochaete chrysosporium)

recombinant, expressed in corn, lyophilized powder, ≥200 units/g solid

Sinónimos:

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium), Manganese-dependent lignin peroxidase, MnP, Peroxidase, manganese

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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recombinant

expressed in corn

Quality Level

form

lyophilized powder

specific activity

≥200 units/g solid

mol wt

~53 kDa (single isozyme)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C (−15°C to −25°C)

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General description

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium) is a lignin-modifying peroxidase that catalyzes the oxidation of Mn2+ to Mn3+. Mn3+ is stabilized by chelators like oxalic acid. Manganese peroxidase uses peroxide as an oxidant and manganese coordination compounds as charge-transfer mediators.

Manganese peroxidase has a molecular weight of 40-50 kDa and is a glycosylated heme protein.[1][2]

Unit Definition

One unit is the amount of enzyme which will oxidize 1.0 μmole Mn2+ per minute to Mn3+ at pH 4.5 and 25 °C

Physical form

Supplied as a lyophilized powder containing ~50% sodium tartrate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Visite la Librería de documentos

Review: lignin conversion by manganese peroxidase (MnP)
Martin H
Enzyme and Microbial Technology, 30(4) (2002)
Kinetic Analysis of Manganese Peroxidase
I-Ching Kuan
The Journal of Biological Chemistry, 268 (1993)

Questions

1–2 of 2 Questions  
  1. Do you have a protocol that you recommend for the analysis of manganese peroxidase activity?

    1 answer
    1. The Technical Service team can provide the requested documentation for the analysis of manganese peroxidase activity. Please visit https://www.sigmaaldrich.com/techservice and click on "Product Documentation" under the Products Section to submit the required information for further assistance.

      Helpful?

  2. 해당 효소의 유전자 서열과 균주의 strain을 알고 싶습니다

    1 answer
    1. The gene sequence is considered proprietary. The species is Phanerochaete chrysosporium. This is a recombinant protein expressed in corn.

      Helpful?

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