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Merck

70540

Sigma-Aldrich

Naphthol Yellow S

for microscopy (Hist.), for the precipitation (of amino acids and peptides)

Sinónimos:

2,4-Dinitro-1-naphthol-7-sulfonic acid sodium salt, 5,7-Dinitro-8-hydroxy-2-naphthalenesulfonic acid sodium salt, Acid Yellow 1, Flavianic acid sodium salt

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About This Item

Fórmula empírica (notación de Hill):
C10H4N2Na2O8S
Número de CAS:
Peso molecular:
358.19
Colour Index Number:
10316
Beilstein/REAXYS Number:
3839220
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy (Hist.)
for the precipitation (of amino acids and peptides)

form

powder

solubility

methanol: water (1:1): 0.5 g/10 mL, clear

εmax

≥275 at 423-433 nm in water
≥≥ 250 at 387-397 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(cc([N+]([O-])=O)c2ccc(cc12)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C10H6N2O8S.2Na/c13-10-7-3-5(21(18,19)20)1-2-6(7)8(11(14)15)4-9(10)12(16)17;;/h1-4,13H,(H,18,19,20);;/q;2*+1/p-2

InChI key

CTIQLGJVGNGFEW-UHFFFAOYSA-L

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Application

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

Biochem/physiol Actions

Naphthol yellow S (NYS) is used as a stain for protein basic groups. It is an acidic dye and forms a NYS-protein complex at acidic pH. Combination of feulgen and NYS provides DNA to protein ratio.

Analysis Note

λmax. ∼428 nm/∼392 nm; E(1%,1 cm) ∼275-425/∼250-400 (H2O)

Pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1 - STOT RE 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Los clientes también vieron

Scholtissek C, et al.
Chemistry and Cytochemistry of Nucleic Acids and Nuclear Proteins (2012)
W M Frederiks et al.
Histochemistry, 68(1), 49-53 (1980-01-01)
The purpose of this study is to compare the protein content of parenchymal and non-parenchymal nuclei, as isolated from rat liver. The nuclei have been separated by means of a 1 g-sedimentation technique. The protein content of the separated nuclei
J Tas et al.
Acta histochemica. Supplementband, 20, 69-73 (1979-01-01)
After staining with Naphthol Yellow S (NYS) at optimal conditions of pH (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye
N W Schipper et al.
Laboratory investigation; a journal of technical methods and pathology, 61(2), 228-234 (1989-08-01)
The paper describes an improved segmentation method to measure the percentages of epithelium and stroma in ovarian (tumor) tissue with automated image analysis and evaluates its prognostic value. In the image processing method, a blue-yellow image pair is recorded from
Cell and Tissue Culture in Forestry (2012)

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