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00379

Sigma-Aldrich

Atto 495 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

MDL number:
MFCD06798157
UNSPSC Code:
12352108
PubChem Substance ID:
329747054
NACRES:
NA.32

product line

BioReagent

assay

≥90% (HPLC)
≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

λ

in acetonitrile (with 0.1% acetic acid)

UV absorption

λ: 496-502 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(=O)ON4C(=O)CCC4=O)c2c1)=[N+](\C)C

InChI

1S/C25H29N4O4.ClHO4/c1-26(2)19-9-7-17-14-18-8-10-20(27(3)4)16-22(18)28(21(17)15-19)13-5-6-25(32)33-29-23(30)11-12-24(29)31;2-1(3,4)5/h7-10,14-16H,5-6,11-13H2,1-4H3;(H,2,3,4,5)/q+1;/p-1

InChI key

QYNYOHCMTHAQIE-UHFFFAOYSA-M

Application

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient stoke′s shift (excitation maximum 495 nm, emission maximum 527 nm). Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Other Notes

Incorporation in a zeolite photonic nanodevice

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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