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P8136

Sigma-Aldrich

Poli(vinil alcohol)

87-90% hydrolyzed, average mol wt 30,000-70,000

Sinónimos:

PVA, PVAl, PVOH, Poly(Ethenol)

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About This Item

Fórmula lineal:
[-CH2CHOH-]n
Número de CAS:
9002-89-5
MDL number:
MFCD00081922
UNSPSC Code:
12352104
NACRES:
NA.21
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Quality Level

200

form

powder

mol wt

average mol wt 30,000-70,000

viscosity

4-6 cP, 4 % in H2O(20 °C)(lit.)

solubility

H2O: soluble

InChI

1S/C2H4O/c1-2-3/h2-3H,1H2

InChI key

IMROMDMJAWUWLK-UHFFFAOYSA-N

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Application

El poli(vinil alcohol) se ha utilizado:
  • en la producción in vitro (IVP) de embriones de bovino[1]
  • , la recuperación de ovocitos y la maduración in vitro[2]
  • como componente macromolecular en medio de cultivo basal[3]

Biochem/physiol Actions

El poli(vinil alcohol) (PVA) es un polímero polihidroxi soluble en agua. Se sabe que el PVA posee una gran fuerza mecánica, biocompatibilidad y no toxicidad. Por consiguiente, sirve como material de implante biomédico.[4] Polimerización del vinil acetato al poli (vinil acetato), que luego se hidroliza para formar PVA.[5] Su aplicación se observa en los sistemas de administración de fármacos, las vendas para heridas, las membranas de diálisis, la piel artificial, las reparaciones quirúrgicas y los dispositivos cardiovasculares.[4]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000423917
0000423917
0000411485
0000411485
0000411484

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Birth of Piglets Derived from Porcine Zygotes Cultured in a Chemically Defined Medium
Yoshioka K, et al.
Biology of Reproduction, 66(1), 112?119-112?119 (2002)
Structure and applications of poly (vinyl alcohol) hydrogels produced by conventional crosslinking or by freezing/thawing methods
Biopolymers PVA Hydrogels Anionic Polymerisation Nanocomposites, 37-65 (2000)
In Vitro Developmental Competence of In Vitro-Matured Bovine Oocytes Fertilized and Cultured in Completely Defined Media
Keskintepe L and Brackett BG
Biology of Reproduction, 55, 333-339 (1996)
Nanostructured poly(vinyl alcohol)/bioactive glass and poly(vinyl alcohol)/chitosan/bioactive glass hybrid scaffolds for biomedical applications
Mansur HS and Costa HS
Chemical Engineering Journal, 137, 72-83 (2008)
C Wrenzycki et al.
Human reproduction (Oxford, England), 16(5), 893-901 (2001-05-02)
Assisted reproduction technologies have made great progress during the last 15 years in most mammalian species, including humans. Growing evidence indicates that bovine pre-implantation development is a superior model for investigating early human development than the mouse. The purpose of
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Questions

1–10 of 11 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, once polyvinyl alcohol has been dissolved in water, how long can it last and at what temperature should the reconstituted reagent be stored at?

    1 answer

    1. The stability of this product in solution after reconstituted is not determined. It is recommended to use freshly prepared solutions.

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  4. Does poly(vinyl alcohol) have a shelf life?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

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  5. What is the MW of this product if Molarity of the solution is a requirement for fiber fabrication? Is it Average of the given values?

    1 answer

    1. The exact molecular weight will be on the lot-specific Certificate of Analysis. Please see the sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P8136-1KG0000231138.pdf

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  6. Can I sterile-filter a solution of this product?

    1 answer

    1. In general, PVA solutions are very viscous, and sterile-filtering would probably be quite difficult.  It might be better to coat the surface of interest and then irradiate the coated surface under UV light, such as in a cell culture hood.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. Can I use this product in cell culture work?

    1 answer

    1. We have not specifically tested P8136 for use with cultured cells.  This is also not a sterile product. However, several publications claim use of P8136 in culture work.

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  9. What does "87-90% hydrolyzed" mean?

    1 answer

    1. Polyvinyl alcohol is normally prepared by hydrolysis of the acetate, CH3(C=O)O, groups of polyvinylacetate, to leave behind the polyvinyl backbone with the OH groups.  The expected degree of acetate hydrolysis is 87-90%.  This means that 87-90% of the side groups are OH, while the remaining 10-13% side groups are CH3(C=O)O.

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  10. What is the best way to prepare a solution of Product P8136, poly(vinyl alcohol)?

    1 answer

    1. Product P8136, poly(vinyl alcohol), should be solubilized in cold water with adequate agitation to ensure complete wetting of the particles. (That is, keep the particles suspended with gentle stirring.) The temperature should then be increased to 85°C for 30 minutes to ensure complete dissolution. Product P8136 has been found to be soluble in water at a concentration of 50 mg/mL, yielding a clear to slightly hazy, colorless to faint yellow solution.

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1–10 of 11 Questions  

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