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Merck
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Key Documents

63011

Millipore

M9 Broth

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

form

powder

composition

ammonium chloride, 1 g/L
disodium hydrogen phosphate, 6 g/L
potassium dihydrogen phosphate, 3 g/L
sodium chloride, 0.5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

food and beverages
life science and biopharma
microbiology

suitability

nonselective for Escherichia coli
nonselective for coliforms

Application

M9 Broth is used as a microbiological growth media and for the preparation of phage and plasmid DNA using E. coli K12 strains, acc. to Miller (1987).

Preparation Note

Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140)

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCL5188
BCCK5251
BCCJ5499
BCCG8471
BCCD5614

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Khaled M Khleifat et al.
Current microbiology, 57(4), 364-370 (2008-07-30)
A successful attempt was made to isolate linear alkylbenzene sulfonate (LAS)-degrading bacteria from soil irrigated with wastewater. The isolated bacteria were able to use LAS as sole carbon and energy source. Maximum growth rates on LAS reached only 0.27 h(-1).
D Pérez-Conesa et al.
Journal of food protection, 74(1), 55-62 (2011-01-12)
Carvacrol and eugenol were encapsulated in micellar nonionic surfactant solutions to increase active component concentrations in the aqueous phase and used to treat two strains of Listeria monocytogenes (Scott A and 101) and two strains of Escherichia coli O157:H7 (4388
Pyoung Il Kim et al.
Journal of microbiology and biotechnology, 20(1), 138-145 (2010-02-06)
A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30degreesC. Biosurfactant lipopeptides produced by Bacillus
Nancy Valentine et al.
Applied and environmental microbiology, 71(1), 58-64 (2005-01-11)
Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive
Practical aspects of preparing phage and plasmid DNA: growth, maintenance, and storage of bacteria and bacteriophage.
H Miller
Methods in enzymology, 152, 145-170 (1987-01-01)

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