NGLYF-RO
Roche
N-Glycosidase F
PNGase F of Flavobacterium meningosepticum, recombinant from E. coli
Sinónimos:
glycosidase
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About This Item
Productos recomendados
recombinante
expressed in E. coli
Nivel de calidad
conjugado
(N-linked)
Ensayo
≥90% (SDS-PAGE)
Formulario
solution
actividad específica
~25000 units/mg protein
envase
pkg of 0.1 mL (11365169001 [100 U])
pkg of 0.25 mL (11365177001 [250 U])
fabricante / nombre comercial
Roche
pH óptimo
7.0-8.0
temp. de almacenamiento
−20°C
Descripción general
Peptide-N-glycosidase F, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase
Aplicación
Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.
Definición de unidad
One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.
Forma física
Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2
Otras notas
For life science research only. Not for use in diagnostic procedures.
Información legal
The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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Protocolos
N-Glycosidase F Protocol & Troubleshooting
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