10837059001
Roche
Lysozyme
from hen egg white
Sinónimos:
Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
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About This Item
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origen biológico
chicken egg white
Nivel de calidad
Ensayo
≥95%
Formulario
solid
actividad específica
>23000 U/mg (Shugar units)
mol peso
14.4 kDa
envase
pkg of 10 g
fabricante / nombre comercial
Roche
pH óptimo
6.0-7.0
Absorción
3.9 at 280 nm (10 mg enzyme/ml)
Descripción general
Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase
Aplicación
Lysozyme has been used for:
- Cell wall degradation[1]
- Preparation of protoplasts[2]
- Bacteriolysis[3]
- Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
- Food and drinks (flavor enhancer)
- Sample preparation prior to isolation of nucleic acids[4]
Acciones bioquímicas o fisiológicas
Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.[7]
Definición de unidad
One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.
Nota de preparación
Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Reconstitución
Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.
Almacenamiento y estabilidad
Store at 2 to 8 °C. (Store dry!)
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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